PRODUCT CODE: ER1706-24

ROBO1 Antibody (ER1706-24)

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

ICC staining ROBO1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ROBO1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ROBO1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ROBO1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
ICC staining ROBO1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ROBO1 Antibody (ER1706-24)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, MCF-7, A549, human liver cancer tissue, human breast tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

180 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

ROBO1

SYNONYMS

Deleted in U twenty twenty antibody; DUTT 1 antibody; DUTT1 antibody; FLJ21882 antibody; H Robo 1 antibody; H-Robo-1 antibody; hRobo 1 antibody; Robo 1 antibody; Robo1 antibody; ROBO1_HUMAN antibody; Roundabout 1 antibody; Roundabout axon guidance receptor homolog 1 antibody; Roundabout homolog 1 antibody; Roundabout homolog1 precurser antibody; Roundabout1 antibody; SAX 3 antibody; SAX3 antibody

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. ROBO family.

TISSUE SPECIFICITY

Widely expressed, with exception of kidney.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated. May be deubiquitinated by USP33.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Specific cells in the midline separate the left and right halves of the central nervous system and play many roles in guiding the growth cone behavior. In vertebrate spinal cord, insect abdominal nerves and nematodes, midline cells produce induced cues, such as nectins and slits, respectively, as attractants and repellents. respectively. These cells can serve as gatekeepers to prevent axons from passing through the midline and to guide the growth cone in response to the switching of lead cues beyond the crossing. One such gatherer, Robo, is an axon guidance receptor that defines a new subfamily of proteins from fruit flies to mammalian conserved Ig superfamily proteins. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous manner Non-intersecting axons express high levels of Robo and cross axons to express low levels of Robo and then reach midline and high levels of crossover. Robo1 and Robo2 are two human homologs of Drosophila megalopolis. Robo1 is also homologous to the C. elegans gene sax3, while Robo2 is homologous to zebrafish genes.