PRODUCT CODE: ER1902-12

RIP3 Antibody (ER1902-12)

Applications

  • WB

REACTIVITY

  • Mouse

  • Rat

Western blot analysis of RIP3 on MEF cell lysates . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-12, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control:<br />
 Lane 1: Wild-type MEF whole cell lysate (20 µg)<br />
 Lane 2: RIP3 knockout MEF whole cell lysate (20 µg)
  • Western blot analysis of RIP3 on MEF cell lysates . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-12, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control:<br />
 Lane 1: Wild-type MEF whole cell lysate (20 µg)<br />
 Lane 2: RIP3 knockout MEF whole cell lysate (20 µg)
Western blot analysis of RIP3 on MEF cell lysates . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-12, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Wild-type MEF whole cell lysate (20 µg)
Lane 2: RIP3 knockout MEF whole cell lysate (20 µg)

Applications

  • WB

REACTIVITY

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

RIP3 Antibody (ER1902-12)

Immunogen

Synthetic peptide within c terminal mouse rip3.

Host

Rabbit

Positive Control

MEF cell lysates.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

57 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

RIP3

SYNONYMS

Receptor interacting protein 3 antibody; Receptor interacting serine threonine kinase 3 antibody; Receptor interacting serine/threonine protein kinase 3 antibody; Receptor-interacting protein 3 antibody; Receptor-interacting serine/threonine-protein kinase 3 antibody; RIP 3 antibody; RIP like protein kinase 3 antibody; RIP-3 antibody; RIP-like protein kinase 3 antibody; RIPK 3 antibody; RIPK3 antibody; RIPK3_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

TISSUE SPECIFICITY

Expressed in embryo and in adult spleen, liver, testis, heart, brain and lung.

POST-TRANSLATIONAL MODIFICATION

RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-204 plays a role in the necroptotic function of RIPK3. Phosphorylation at Ser-232 is required for binding MLKL. Phosphorylation at Thr-187 is important for its kinase activity, interaction with PELI1 and for its ability to mediate TNF-induced necroptosis (By similarity).; Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B. Ubiquitinated by STUB1 leading to its subsequent proteasome-dependent degradation.

SUBCELLULAR LOCATION

Mitochondrion, Cytosol, Plasma membrane.

FUNCTION

Receptor-interacting serine/threonine-protein kinase 3 is an enzyme that in humans is encoded by the RIPK3 gene. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.