PRODUCT CODE: ET1706-18

Recombinant Zyxin Monoclonal Antibody (ET1706-18)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.
  • Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.
  • ICC staining Zyxin in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Zyxin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Zyxin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Zyxin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Zyxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Zyxin Monoclonal Antibody (ET1706-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, LOVO, PC-3M, human kidney tissue, mouse smooth muscle tissue,Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU83-12

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75/61/45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Zyxin

SYNONYMS

ESP 2 antibody; ESP2 antibody; HED 2 antibody; HED2 antibody; ZYX antibody; ZYX protein antibody; Zyxin 2 antibody; Zyxin2 antibody

SEQUENCE SIMILARITIES

Belongs to the zyxin/ajuba family.

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton and Nucleus.

FUNCTION

Zyxin is a low abundance phosphoprotein localized to focal adhesion plaques and is thought to perform regulatory functions at these regions. The protein contains a number of proline-rich sequences as well as three LIM domains, zinc finger domains involved in protein binding. Zyxin interacts with several other proteins at cell adhesion sites, including members of the CRP (cysteine-rich protein) LIM domain containing protein family. The proline-rich domain of Zyxin associates with an SH3 domain of p95 Vav, but not with similar SH3 domains containing proteins such as GRB2 or PLCg. Zyxin has also been shown to interact with the focal adhesion protein VASP and may assist in the targeting of VASP to focal adhesions, microfilaments and membrane regions of high dynamic activity.