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PRODUCT CODE: ET1706-18

Recombinant Zyxin Monoclonal Antibody (ET1706-18)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.
  • Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.
  • ICC staining Zyxin in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Zyxin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Zyxin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Zyxin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Zyxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Zyxin Monoclonal Antibody (ET1706-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, LOVO, PC-3M, human kidney tissue, mouse smooth muscle tissue,Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU83-12

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75/61/45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q15942

PROTEIN NAME

Zyxin

GENE NAME

ZYX

SYNONYMS

ZYX

SEQUENCE SIMILARITIES

Belongs to the zyxin/ajuba family.

SUBCELLULAR LOCATION

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Note=Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.

FUNCTION

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).