Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
FC
-
IP
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Zyxin Monoclonal Antibody (ET1706-18)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
MCF-7, LOVO, PC-3M, human kidney tissue, mouse smooth muscle tissue,Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU83-12
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
75/61/45 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Zyxin
SYNONYMS
ESP 2 antibody; ESP2 antibody; HED 2 antibody; HED2 antibody; ZYX antibody; ZYX protein antibody; Zyxin 2 antibody; Zyxin2 antibody
SEQUENCE SIMILARITIES
Belongs to the zyxin/ajuba family.
SUBCELLULAR LOCATION
Cytoplasm, cytoskeleton and Nucleus.
FUNCTION
Zyxin is a low abundance phosphoprotein localized to focal adhesion plaques and is thought to perform regulatory functions at these regions. The protein contains a number of proline-rich sequences as well as three LIM domains, zinc finger domains involved in protein binding. Zyxin interacts with several other proteins at cell adhesion sites, including members of the CRP (cysteine-rich protein) LIM domain containing protein family. The proline-rich domain of Zyxin associates with an SH3 domain of p95 Vav, but not with similar SH3 domains containing proteins such as GRB2 or PLCg. Zyxin has also been shown to interact with the focal adhesion protein VASP and may assist in the targeting of VASP to focal adhesions, microfilaments and membrane regions of high dynamic activity.