PRODUCT CODE: ET1706-42

Recombinant ZAP70 Monoclonal Antibody (ET1706-42)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

Western blot analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution.
  • Western blot analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution.
  • ICC staining ZAP70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ZAP70 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ZAP70 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with ZAP70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant ZAP70 Monoclonal Antibody (ET1706-42)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human thymus tissue lysate, LOVO, SH-SY5Y, human tonsil tissue, human spleen tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU08-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

ZAP70

SYNONYMS

70 kDa zeta associated protein antibody; 70 kDa zeta-associated protein antibody; EC 2.7.10.2 antibody; FLJ17670 antibody; FLJ17679 antibody; Selective T cell defect antibody; SRK antibody; STD antibody; Syk related tyrosine kinase antibody; Syk-related tyrosine kinase antibody; Truncated ZAP kinase antibody; Tyrosine protein kinase ZAP70 antibody; Tyrosine-protein kinase ZAP-70 antibody; TZK antibody; ZAP 70 antibody; ZAP70 antibody; ZAP70_HUMAN antibody; Zeta chain associated protein kinase 70kD antibody; Zeta chain associated protein kinase 70kDa antibody; Zeta chain associated protein kinase 70kDa isoform 1 antibody; Zeta chain associated protein kinase 70kDa isoform 2 antibody; Zeta chain of T cell receptor associated protein kinase 70 antibody; Zeta chain TCR associated protein kinase 70kD antibody; Zeta chain TCR associated protein kinase 70kDa antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.

TISSUE SPECIFICITY

Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22.; Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.

SUBCELLULAR LOCATION

Cytoplasm. Cell membrane.

FUNCTION

The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and e chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation.