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PRODUCT CODE: ET1609-10

Recombinant YB1 Monoclonal Antibody (ET1609-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: Jurkat
  • Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Hela <br /> Lane 2: Jurkat
  • ICC staining YB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining YB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining YB1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-YB1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant YB1 Monoclonal Antibody (ET1609-10)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, MCF-7, Hela, HepG2, human breast carcinoma tissue, human kidney tissue, mouse colon tissue, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST0432

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P67809

PROTEIN NAME

YB1

SYNONYMS

BP 8 antibody; CBF-A antibody; CCAAT binding transcription factor I subunit A antibody; CCAAT-binding transcription factor I subunit A antibody; CSDA2 antibody; CSDB antibody; DBPB antibody; DNA binding protein B antibody; DNA-binding protein B antibody; EFI-A antibody; Enhancer factor I subunit A antibody; MDR NF1 antibody; MGC104858 antibody; MGC110976 antibody; MGC117250 antibody; NSEP 1 antibody; NSEP1 antibody; Nuclease sensitive element binding protein 1 antibody; Nuclease-sensitive element-binding protein 1 antibody; p50 antibody; Q15905 antibody; Y-box binding protein 1 antibody; Y-box transcription factor antibody; Y-box-binding protein 1 antibody; YB 1 antibody; YB-1 antibody; YBOX1_HUMAN antibody; YBX 1 antibody; ybx1 antibody

SEQUENCE SIMILARITIES

Belongs to the YBX1 family.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcactivational ability.; In the absence of phosphorylation the protein is retained in the cytoplasm.; Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus (By similarity).

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cytoplasmic granule, Secreted.

FUNCTION

Y-box binding protein YB-1 (also known as CCAAT-binding transcription factor, enhancer factor I subunit A and DNA-binding protein B) belongs to a family of multifunctional proteins which regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing Arginine clusters and aromatic residues. YB-1 plays a role in cell proliferation as an activator of growth-associated gene expression. YB-1 is also a repressor of the cell death-associated gene FAS. YB-1 may play an important role in controlling cell survival by regulating the expression of cell growth-associated and death-associated genes.