Recombinant Rabbit monoclonal primary
Recombinant YAP1 Monoclonal Antibody (ET1608-30)
HepG2 cell lysates, Hela, HepG2, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, NIH/3T3.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
65 kDa Yes associated protein antibody; 65 kDa Yes-associated protein antibody; COB1 antibody; YAp 1 antibody; YAP 65 antibody; YAP antibody; YAP1 antibody; YAP1_HUMAN antibody; YAP2 antibody; YAP65 antibody; yes -associated protein delta antibody; Yes associated protein 1 65kDa antibody; Yes associated protein 1 antibody; Yes associated protein 2 antibody; yes associated protein beta antibody; YKI antibody; Yorkie homolog antibody
Belongs to the YAP1 family.
Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation. Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1.; Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase.
Commitment to cell division occurs at a point late in the G1 phase of the cell cycle, termed Start. Passage through Start requires the activation of the Cdc28 protein kinase by the cell cycle-regulated G1 cyclins. Maximal expression of these G1 cyclins is induced by the heterodimeric transcription factor complex composed of Swi4 (also designated Art1) and Swi6. Swi4 is the DNA-binding subunit of this complex. In addition to binding Swi4, Swi6 forms a complex with Mbp1. This complex activates S-phase cyclins and genes involved in DNA synthesis Rpb1 is the largest subunit of the yeast RNA polymerase II. Srb4 is a basal transcription factor that is essential for the establishment of the transcription initiation apparatus. Stress factors induce transcription through the induction of various transcription factors. Yap1 activates expression in response to oxidative stress, while Msn2 and Msn4 mediate transcription via the stress response element (STRE).