PRODUCT CODE: ET1704-01

Recombinant XRCC1 Monoclonal Antibody (ET1704-01)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of XRCC1 on different cells lysates using anti-XRCC1 antibody at 1/500 dilution.<br />
 Positive control: <br />
  Line 1: Hela <br />
  Line 2: 293T <br />
  Line 3: Jarkat
  • Western blot analysis of XRCC1 on different cells lysates using anti-XRCC1 antibody at 1/500 dilution.<br />
 Positive control: <br />
  Line 1: Hela <br />
  Line 2: 293T <br />
  Line 3: Jarkat
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- XRCC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast infiltrating duct carcinoma tissue using anti- XRCC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- XRCC1 antibody. Counter stained with hematoxylin.
Western blot analysis of XRCC1 on different cells lysates using anti-XRCC1 antibody at 1/500 dilution.
Positive control:
Line 1: Hela
Line 2: 293T
Line 3: Jarkat

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant XRCC1 Monoclonal Antibody (ET1704-01)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, 293T, Hela, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA11-47

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100/70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

XRCC1

SYNONYMS

DNA repair protein XRCC1 antibody; RCC antibody; X ray repair complementing defective repair in chinese hamster antibody; X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody; X ray repair complementing defective repair in chinese hamster cells 1 antibody; X ray repair cross complementing 1 antibody; X ray repair cross complementing protein 1 antibody; X ray repair, complementing defective, repair in Chinese hamster antibody; X ray repair cross-complementing protein 1 antibody; XRCC 1 antibody; Xrcc1 antibody; XRCC1_HUMAN antibody

TISSUE SPECIFICITY

Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.; Sumoylated.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad5. XRCC1 is a protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.