PRODUCT CODE: ET1704-01

Recombinant XRCC1 Monoclonal Antibody (ET1704-01)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of XRCC1 on different cells lysates using anti-XRCC1 antibody at 1/500 dilution.<br />
Positive control: <br />
 Line 1: Hela <br />
 Line 2: 293T <br />
 Line 3: Jarkat
  • Western blot analysis of XRCC1 on different cells lysates using anti-XRCC1 antibody at 1/500 dilution.<br />
Positive control: <br />
 Line 1: Hela <br />
 Line 2: 293T <br />
 Line 3: Jarkat
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- XRCC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast infiltrating duct carcinoma tissue using anti- XRCC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- XRCC1 antibody. Counter stained with hematoxylin.
Western blot analysis of XRCC1 on different cells lysates using anti-XRCC1 antibody at 1/500 dilution.
Positive control:
Line 1: Hela
Line 2: 293T
Line 3: Jarkat

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant XRCC1 Monoclonal Antibody (ET1704-01)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, 293T, Hela, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA11-47

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100/70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

DNA repair protein XRCC1

GENE NAME

XRCC1

SYNONYMS

XRCC1

TISSUE SPECIFICITY

Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.; Sumoylated.

SUBCELLULAR LOCATION

Nucleus. Note=Moves from the nucleoli to the global nuclear chromatin upon DNA damage.

FUNCTION

Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.