PRODUCT CODE: ET1706-33

Recombinant Wnt5a Monoclonal Antibody (ET1706-33)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Wnt5a on Hela cell lysate using anti-Wnt5a antibody at 1/1,000 dilution.
  • Western blot analysis of Wnt5a on Hela cell lysate using anti-Wnt5a antibody at 1/1,000 dilution.
  • ICC staining Wnt5a in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Wnt5a antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with Wnt5a antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Wnt5a on Hela cell lysate using anti-Wnt5a antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Wnt5a Monoclonal Antibody (ET1706-33)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, PC-3M, human lung cancer tissue, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU30-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • ICC

  • 1:50 FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Wnt5a

SYNONYMS

hWNT 5A antibody; hWNT5A antibody; Protein Wnt 5a antibody; Protein Wnt-5a antibody; Protein Wnt5a antibody; Wingless type MMTV integration site family member 5A antibody; Wnt 5a antibody; WNT 5A protein antibody; WNT 5A protein precursor antibody; WNT5A antibody; WNT5A protein precursor antibody; WNT5A_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the Wnt family.

TISSUE SPECIFICITY

Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level). Detected in neonate heart and lung.

POST-TRANSLATIONAL MODIFICATION

Glycosylation is necessary for secretion but not for activity.; Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.; Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.

SUBCELLULAR LOCATION

Secreted, extracellular space, extracellular matrix.

FUNCTION

The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene "wingless" and to its vertebrate ortholog, Int1, a mouse protooncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins, which have features typical of secreted growth factors. Northern blot analysis detects expression of Wnt-5a in brain, lung, and heart. At least five distinct Wnt-5a transcripts are observed, which are due to transcript variability 5-prime to the initiation methionine. In situ hybridization detects a complex spatial and temporal pattern of Wnt-5a in the mouse, including expression in the developing central nervous system, limbs, facial processes, and the posterior region of the fetus. Human frizzled-5 is the receptor for the Wnt-5a ligand. It is suggested that Wnt-5a augments primitive hematopoietic development in vivo and represents an in vivo regulator of hematopoietic stem cell function in the human.

CITATIONS

  • Jiang, Shaojie et al.

    WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling. | Cell Communication and Signaling : Ccs [2019]