PRODUCT CODE: ET7107-20

Recombinant Wnt2b Monoclonal Antibody (ET7107-20)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.
  • Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.
  • ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Wnt2b Monoclonal Antibody (ET7107-20)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SiHa, Hela, LOVO, SHSY-5Y, human kidney tissue, mouse kidney tissue, rat testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB48-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

44 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Protein Wnt-2b

GENE NAME

WNT2B

SYNONYMS

WNT2B, WNT13

SEQUENCE SIMILARITIES

Belongs to the Wnt family.

TISSUE SPECIFICITY

Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.

POST-TRANSLATIONAL MODIFICATION

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

SUBCELLULAR LOCATION

Secreted, extracellular space, extracellular matrix.

FUNCTION

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.