PRODUCT CODE: ET1705-60

Recombinant WDR5 Monoclonal Antibody (ET1705-60)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of WDR5 on different cell lysates using anti-WDR5 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Hela Lane 2: A549 Lane 3: NIH-3T3 Lane 4: PC-12
  • Western blot analysis of WDR5 on different cell lysates using anti-WDR5 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Hela Lane 2: A549 Lane 3: NIH-3T3 Lane 4: PC-12
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-WDR5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with WDR5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of WDR5 on different cell lysates using anti-WDR5 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela Lane 2: A549 Lane 3: NIH-3T3 Lane 4: PC-12

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant WDR5 Monoclonal Antibody (ET1705-60)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, A549, NIH-3T3, PC-12, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM73-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

WD repeat-containing protein 5

GENE NAME

WDR5

SYNONYMS

WDR5, BIG3

SEQUENCE SIMILARITIES

Belongs to the WD repeat WDR5/wds family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.