PRODUCT CODE: ET1610-69

Recombinant WASF2 Monoclonal Antibody (ET1610-69)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of WASF2 on human placenta lysates using anti-WASF2 antibody at 1/1,000 dilution.
  • Western blot analysis of WASF2 on human placenta lysates using anti-WASF2 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-WASF2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-WASF2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-WASF2 antibody. Counter stained with hematoxylin.
Western blot analysis of WASF2 on human placenta lysates using anti-WASF2 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant WASF2 Monoclonal Antibody (ET1610-69)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue lysates, human kidney tissue, mouse kidney tissue, mouse placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC66-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

WASF2

SYNONYMS

DICTYOSTELIUM antibody; dJ393P12.2 antibody; IMD2 antibody; Protein WAVE-2 antibody; Putative Wiskott Aldrich syndrome protein family member 4 antibody; SCAR 2 antibody; SCAR antibody; SCAR2 antibody; Suppressor of cyclic-AMP receptor (WASP family) antibody; Verprolin homology domain-containing protein 2 antibody; WASF2 antibody; WASF2_HUMAN antibody; WASF4 antibody; WASP family protein member 2 antibody; WASP family protein member 4 antibody; WASP family Verprolin homologous protein 2 antibody; WASP-family protein member 2 antibody; WAVE 2 antibody; WAVE2 antibody; Wiskott-Aldrich syndrome protein family member 2 antibody; Wiskott-Aldrich syndrome protein family verprolin-homologous protein antibody

SEQUENCE SIMILARITIES

Belongs to the SCAR/WAVE family.

TISSUE SPECIFICITY

Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.

SUBCELLULAR LOCATION

Basolateral cell membrane, cytoskeleton, lamellipodium.

FUNCTION

WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in Actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the Actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating Actin reorganization downstream of the Rho family of small GTPases. The protein homologs WAVE1 and WAVE2 regulate membrane ruffling by inducing the formation of Actin filament clusters in response to GTP binding and by activating Rac. They mediate Actin polymerization by cooperating with the Arp2/3 complex, thereby promoting the formation of Actin filaments. WAVE1, which is also designated SCAR (suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed, with the expression highest in peripheral blood leukocytes. WAVE3 forms a multiprotein complex that links receptor kinases with Actin and plays a role in the transduction of signals involving changes in cell shape, function or motility.