PRODUCT CODE: ET7107-01

Recombinant VPS35 Monoclonal Antibody (ET7107-01)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
 Lane 2: U937<br />
 Lane 3: SH-SY-5Y<br />
  • Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
 Lane 2: U937<br />
 Lane 3: SH-SY-5Y<br />
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3M
Lane 2: U937
Lane 3: SH-SY-5Y

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant VPS35 Monoclonal Antibody (ET7107-01)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-3M, U937, SH-SY-5Y, human colon cancer tissue, human kidney tissue, human placenta tissue, mouse colon tissue, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB33-82

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

91 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:100-1:300

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Vacuolar protein sorting-associated protein 35

GENE NAME

VPS35

SYNONYMS

hVPS35, VPS35, MEM3, TCCCTA00141

SEQUENCE SIMILARITIES

Belongs to the VPS35 family.

TISSUE SPECIFICITY

Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.

SUBCELLULAR LOCATION

Cytoplasm. Membrane; Peripheral membrane protein. Endosome. Early endosome.

FUNCTION

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of WASHC2C. Mediates the association of the CSC with the WASH complex via WASHC2. Required for the endosomal localization of TBC1D5.; (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus.