PRODUCT CODE: ET1609-39

Recombinant Vitronectin Monoclonal Antibody (ET1609-39)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Human liver <br />
 Lane 2: Human serum
  • Western blot analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Human liver <br />
 Lane 2: Human serum
  • ICC staining Vitronectin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Vitronectin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Vitronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
Western blot analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human liver
Lane 2: Human serum

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Vitronectin Monoclonal Antibody (ET1609-39)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, A549, MCF-7, HepG2, mouse liver tissue, mouse skin tissue, human liver tissue, human liver cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST49-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54/75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Vitronectin

GENE NAME

VTN

SYNONYMS

VN, VTN

TISSUE SPECIFICITY

Expressed in the retina pigment epithelium (at protein level). Expressed in plasma (at protein level). Expressed in serum (at protein level).

POST-TRANSLATIONAL MODIFICATION

Sulfated on tyrosine residues.; N- and O-glycosylated.; Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.; It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.; Phosphorylation sites are present in the extracellular medium.

SUBCELLULAR LOCATION

Secreted, extracellular space.; Parasitophorous vacuole. Note=(Microbial infection) In P.falciparum-infected red blood cells, VTN internalization is detected at the early trophozoite stage. Colocalizes with SERA5 at the schizont stage and with SERA5 P47 at the merozoite surface.

FUNCTION

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.; Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.