PRODUCT CODE: ET1703-09

Recombinant Vitamin D Binding protein Monoclonal Antibody (ET1703-09)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.
  • Western blot analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.
  • ICC staining DBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DBP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DBP in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- DBP antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with DBP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Vitamin D Binding protein Monoclonal Antibody (ET1703-09)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SKOV-3, Hela, HepG2, human kidney tissue, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-36

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Vitamin D-binding protein

GENE NAME

GC

SYNONYMS

DBP, VDB, Gc-MAF, Gc, DBP-maf

SEQUENCE SIMILARITIES

Belongs to the ALB/AFP/VDB family.

TISSUE SPECIFICITY

Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.

POST-TRANSLATIONAL MODIFICATION

Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.