PRODUCT CODE: ET1705-94

Recombinant Vinculin Monoclonal Antibody (ET1705-94)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Vinculin on different lysates using anti-Vinculin antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2<br />
 Lane 2: Human fetal skeletal muscle<br />
 Lane 3: Rat spleen<br />
 Lane 4: Mouse kidney
  • Western blot analysis of Vinculin on different lysates using anti-Vinculin antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: HepG2<br />
 Lane 2: Human fetal skeletal muscle<br />
 Lane 3: Rat spleen<br />
 Lane 4: Mouse kidney
  • ICC staining Vinculin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Vinculin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Vinculin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Vinculin on different lysates using anti-Vinculin antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Human fetal skeletal muscle
Lane 3: Rat spleen
Lane 4: Mouse kidney

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Vinculin Monoclonal Antibody (ET1705-94)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2 cell lysate, human skeletal muscle tissue lysate, rat spleen tissue lysate, mouse kidney tissue lysate, A431, A549, HUVEC.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM42-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

124 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Vinculin

SYNONYMS

CMD1W antibody; CMH15 antibody; Epididymis luminal protein 114 antibody; HEL114 antibody; Metavinculin antibody; MV antibody; MVCL antibody; OTTHUMP00000019861 antibody; OTTHUMP00000019862 antibody; VCL antibody; VINC antibody; VINC_HUMAN antibody; Vinculin antibody

SEQUENCE SIMILARITIES

Belongs to the vinculin/alpha-catenin family.

TISSUE SPECIFICITY

Metavinculin is muscle-specific.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity).; Acetylated; mainly by myristic acid but also by a small amount of palmitic acid.

SUBCELLULAR LOCATION

Cell membrane, sarcolemma, cytoskeleton, adherens junction, focal adhesion.

FUNCTION

Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

CITATIONS

  • Xu, Xiu-Ping et al.

    Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro. | Stem Cell Research & Therapy [2017]

  • Li, Junhong et al.

    Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. | OncoTargets and Therapy [2020]