Positive control:
Lane 1: Raji cell lysate
Lane 2: HepG2 cell lysate
Lane 2: SW480 cell lysate
Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant VDAC1 Monoclonal Antibody (ET1601-20)
Immunogen
Synthetic peptide within n-terminal human vdac1.
Host
Rabbit
Positive Control
Raji cell lysate, HepG2 cell lysate, SW480 cell lysate, human liver tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA93-03
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
31 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:5,000
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
VDAC1
SYNONYMS
N2441 antibody; OMP2 antibody; POR1 antibody; hVDAC1 antibody; MGC111064 antibody; Mitochondrial Porin antibody; Outer mitochondrial membrane protein porin 1 antibody; Plasmalemmal porin antibody; Porin 31HL antibody; Porin 31HM antibody; VDAC antibody; VDAC-1 antibody; Vdac1 antibody; VDAC1_HUMAN antibody; Voltage dependent anion channel 1 antibody; Voltage dependent anion selective channel protein 1 antibody; Voltage-dependent anion-selective channel protein 1 antibody; YNL055C antibody; YNL2441C antibody
SEQUENCE SIMILARITIES
Belongs to the eukaryotic mitochondrial porin family.
TISSUE SPECIFICITY
Heart, liver and skeletal muscle.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.; Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
SUBCELLULAR LOCATION
Mitochondrion outer membrane, Cell membrane, Membrane raft
FUNCTION
Voltage-dependent anion-selective channel (VDAC1) (also referred to as porin, isoform 1) is a small protein, originally discovered in the outer membrane of mitochondria where it constitutes the major pore-forming protein. The porin protein VDAC1 allows to the outer-most membrane of the mitochondrion free permeability to low molecular-weight solutes. VDAC1 has been shown to co-immunoprecipitate with the anti-apoptotic protein Bcl-2 and suggested to be involved in forming the mitochondrial pore which releases cytochrome c during apoptosis.