PRODUCT CODE: ET1703-56

Recombinant VCP Monoclonal Antibody (ET1703-56)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution.<br />
 Positive control: Line 1: SH-SY5Y Line 2: HepG2 Line 3: Hela
  • Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution.<br />
 Positive control: Line 1: SH-SY5Y Line 2: HepG2 Line 3: Hela
  • ICC staining VCP in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VCP in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-VCP antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HL-60 cells with VCP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
  • Western blot analysis of VCP on hybrid fish (crucian-carp) heart tissue lysate using anti-VCP antibody at 1/500 dilution.
Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution.
Positive control: Line 1: SH-SY5Y Line 2: HepG2 Line 3: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant VCP Monoclonal Antibody (ET1703-56)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY5Y, MCF-7, HepG2, Hela, SW480, HL-60, human colon cancer tissue, human breast cancer tissue, human liver tissue, rat brain tissue, mouse colon tissue, zebrafish tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-15

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

89 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

VCP

SYNONYMS

15S Mg(2+) ATPase p97 subunit antibody; 15S Mg(2+)-ATPase p97 subunit antibody; ALS14 antibody; ATPase p97 antibody; CDC48 antibody; IBMPFD antibody; MGC131997 antibody; MGC148092 antibody; MGC8560 antibody; p97 antibody; TER ATPase antibody; TERA antibody; TERA_HUMAN antibody; Transitional endoplasmic reticulum ATPase antibody; Valosin containing protein antibody; Valosin-containing protein antibody; VCP antibody; Yeast Cdc48p homolog antibody

SEQUENCE SIMILARITIES

Belongs to the AAA ATPase family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation. Phosphorylated in mitotic cells.; ISGylated.; Methylation at Lys-315 catalyzed by VCPKMT is increased in the presence of ASPSCR1. Lys-315 methylation may decrease ATPase activity.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Valosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-α as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α.