Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: human spleen tissue lysate
Applications
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WB
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ICC
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IF
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IHC-P
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IP
REACTIVITY
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Human
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Mouse
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Rat
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Zebrafish
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant VCAM1 Monoclonal Antibody (ET1601-18)
Immunogen
Synthetic peptide within c-terminal human vcam1.
Host
Rabbit
Positive Control
NIH/3T3 cell lysate, human spleen tissue lysate, HUVEC, human tonsil tissue, human spleen tissue, mouse spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA05-04
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
100 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:500-1:5,000
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ICC/IF
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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assay-dependent
TARGET
UNIPROT #
PROTEIN NAME
VCAM1
SYNONYMS
CD106 antibody; CD106 Antigen antibody; INCAM 100 antibody; INCAM-100 antibody; L1CAM antibody; MGC99561 antibody; V-CAM 1 antibody; Vascular Cell Adhesion Molecule 1 antibody; Vascular cell adhesion protein 1 antibody; VCAM 1 antibody; VCAM-1 antibody; VCAM1 antibody; VCAM1_HUMAN antibody
TISSUE SPECIFICITY
Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
POST-TRANSLATIONAL MODIFICATION
Sialoglycoprotein.
SUBCELLULAR LOCATION
Membrane
FUNCTION
Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms' tumors, Ewing's sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2а1 and B2аM integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM) -like protein and is deleted in 66% of patients with Kallmann's syndrome, anosmia with secondary hypogonadism.
CITATIONS
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Wang, Qianting et al.
Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells. | Archives of Oral Biology [2019]
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WB
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human
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Citation
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