PRODUCT CODE: ET1610-79

Recombinant VAV2 Monoclonal Antibody (ET1610-79)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1,000 dilution.
  • Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1,000 dilution.
  • ICC staining VAV2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAV2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAV2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VAV2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAV2 antibody. Counter stained with hematoxylin.
Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant VAV2 Monoclonal Antibody (ET1610-79)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, SW480, Hela, mouse colon tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC68-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Guanine nucleotide exchange factor VAV2

GENE NAME

VAV2

SYNONYMS

VAV-2, VAV2

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues in response to FGR activation.

FUNCTION

Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity).