PRODUCT CODE: ET1610-79

Recombinant VAV2 Monoclonal Antibody (ET1610-79)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1,000 dilution.
  • Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1,000 dilution.
  • ICC staining VAV2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAV2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAV2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VAV2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAV2 antibody. Counter stained with hematoxylin.
Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant VAV2 Monoclonal Antibody (ET1610-79)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, SW480, Hela, mouse colon tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC68-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

VAV2

SYNONYMS

Guanine nucleotide exchange factor VAV2 antibody; Oncogene VAV2 antibody; Protein vav 2 antibody; VAV 2 antibody; Vav 2 oncogene antibody; VAV-2 antibody; VAV2 antibody; VAV2 oncogene antibody; VAV2_HUMAN antibody

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues in response to FGR activation.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. This protein, known as Vav, Vav1 or p95Vav, contains an N-terminal helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins that, if deleted, causes oncogenic activation. In addition, Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. These results suggest that Vav may represent a new type of signal transduction molecule involved in the transduction of tyrosine phosphorylation signaling into transcriptional events. Vav2 is a member of the Vav family of oncoproteins and acts as a guanosine nucleotide exchange factor (GEF) for RhoG and RhoA-like GTPases in a phosphotyrosine-dependent manner.