PRODUCT CODE: ET1702-10

Recombinant VAMP8 Monoclonal Antibody (ET1702-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: Mouse kidney
  • Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: Mouse kidney
  • Western blot analysis of VAMP8 on U93 cell lysates using anti-VAMP8 antibody at 1/500 dilution.
  • ICC staining VAMP8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAMP8 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAMP8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with VAMP8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse kidney

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant VAMP8 Monoclonal Antibody (ET1702-10)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, 293T, Hela, mouse brain tissue, mouse kidney tissue, human kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0963

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Vesicle-associated membrane protein 8

GENE NAME

VAMP8

SYNONYMS

VAMP-8, EDB, VAMP8

SEQUENCE SIMILARITIES

Belongs to the synaptobrevin family.

TISSUE SPECIFICITY

Platelets.

SUBCELLULAR LOCATION

Lysosome membrane; Single-pass type IV membrane protein; Single-pass type IV membrane protein; Single-pass type IV membrane protein. Cell membrane; Single-pass type IV membrane protein. Note=Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity). Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity). Cycles through the apical but not through the basolateral plasma membrane (By similarity). Apical region of acinar cells; in zymogen granule membranes (By similarity).

FUNCTION

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity).