PRODUCT CODE: ET1703-50

Recombinant VAMP2 Monoclonal Antibody (ET1703-50)

  • Recombinant

Applications

  • WB

  • IP

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of VAMP2 on different cell lysates using anti-VAMP2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: SH-SY5Y<br />
 Lane 2: Jurkat
  • Western blot analysis of VAMP2 on different cell lysates using anti-VAMP2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: SH-SY5Y<br />
 Lane 2: Jurkat
  • ICC staining VAMP2 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAMP2 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAMP2 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of SH-SY5Y cells with VAMP2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of VAMP2 on different cell lysates using anti-VAMP2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: Jurkat

Applications

  • WB

  • IP

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant VAMP2 Monoclonal Antibody (ET1703-50)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, SH-SY5Y, N2A, SHG-44.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-00

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

15 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

VAMP2

SYNONYMS

FLJ11460 antibody; RATVAMPB antibody; RATVAMPIR antibody; SYB antibody; SYB2 antibody; Synaptobrevin 2 antibody; Synaptobrevin-2 antibody; VAMP 2 antibody; VAMP-2 antibody; Vamp2 antibody; VAMP2_HUMAN antibody; Vesicle associated membrane protein 2 antibody; Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody; Vesicle-associated membrane protein 2 antibody

SEQUENCE SIMILARITIES

Belongs to the synaptobrevin family.

TISSUE SPECIFICITY

Nervous system and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which hydrolyzes the 76-Gln-|-Phe-77 bond and probably inhibits neurotransmitter release.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 59-Lys-|-Leu-60 bond and inhibits neurotransmitter release. Note that humans are not known to be infected by C.botulinum type D.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 58-Gln-|-Lys-59 bond and probably inhibits neurotransmitter release.; (Microbial infection) Targeted and hydrolyzed by C.tetani tetanus toxin (tetX) which hydrolyzes the 76-Gln-|-Phe-77 bond and probably inhibits neurotransmitter release.

SUBCELLULAR LOCATION

Cell junction, Cell membrane, Cytoplasmic vesicle , Synapse, Synaptosome.

FUNCTION

Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs, also designated synaptobrevins, including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including a- and γ-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exo-cytosis by competing with SNAP 25 and syntaxins for VAMP binding.