PRODUCT CODE: ET1706-13

Recombinant USP22 Monoclonal Antibody (ET1706-13)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

  • IP

REACTIVITY

  • Human

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Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.
  • Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.
  • ICC staining USP22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP22 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP22 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • FC

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant USP22 Monoclonal Antibody (ET1706-13)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, LOVO, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU63-27

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

USP22

SYNONYMS

Deubiquitinating enzyme 22 antibody; KIAA1063 antibody; Ubiquitin carboxyl terminal hydrolase 22 antibody; Ubiquitin carboxyl-terminal hydrolase 22 antibody; Ubiquitin specific peptidase 22 antibody; Ubiquitin specific peptidase 3 like antibody; Ubiquitin specific processing protease 22 antibody; Ubiquitin specific protease 22 antibody; Ubiquitin thioesterase 22 antibody; Ubiquitin thiolesterase 22 antibody; Ubiquitin-specific-processing protease 22 antibody; UBP22_HUMAN antibody; USP 22 antibody; Usp22 antibody; USP3L antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family. UBP8 subfamily.

TISSUE SPECIFICITY

Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin C-terminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker. The gene encoding USP22 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.