PRODUCT CODE: ET1706-13

Recombinant USP22 Monoclonal Antibody (ET1706-13)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

  • IP

REACTIVITY

  • Human

Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.
  • Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.
  • ICC staining USP22 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP22 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP22 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of USP22 on Hela (1) and HepG2 (2) cell lysate using anti-USP22 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • FC

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant USP22 Monoclonal Antibody (ET1706-13)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, LOVO, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU63-27

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Ubiquitin carboxyl-terminal hydrolase 22

GENE NAME

USP22

SYNONYMS

USP22, KIAA1063, USP3L

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family. UBP8 subfamily.

TISSUE SPECIFICITY

Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.