PRODUCT CODE: ET1701-9

Recombinant Ubiquitin D Monoclonal Antibody (ET1701-9)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1,000 dilution.
  • Western blot analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1,000 dilution.
  • ICC staining Ubiquitin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ubiquitin D in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ubiquitin D in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin.
Western blot analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Ubiquitin D Monoclonal Antibody (ET1701-9)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

F9, Hela, HepG2, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ084-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

18 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Ubiquitin D

SYNONYMS

Diubiquitin antibody; FAT10 antibody; GABBR1 antibody; UBD 3 antibody; Ubd antibody; UBD_HUMAN antibody; Ubiquitin D antibody; Ubiquitin like protein FAT10 antibody; Ubiquitin-like protein FAT10 antibody

TISSUE SPECIFICITY

Constitutively expressed in mature dendritic cells and B-cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland.

POST-TRANSLATIONAL MODIFICATION

Can be acetylated.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

FAT10, also designated Ubiquitin D or Diubiquitin, is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin. Each domain contains two cysteines, along with a free C-terminal diglycine motif required for FAT10 conjugate formation. FAT10 is inducible by interferon-g and tumor necrosis factor a (TNF?). The FAT10 protein interacts with MAD2, a component of the spindle checkpoint, and plays a role in antigen presentation, cytokine response, apoptosis and mitosis. It may also regulate cell growth during dendritic cell or B cell activation and development. FAT10 mRNA is expressed mainly in some dendritic cells and lymphoblastoid lines and in other specific cells subsequent to interferon-g induction. The human FAT10 gene, designated UBD, maps to chromosome 6p21.3 and is overexpressed in the tumors of various epithelial cancers.