Lane 1: HUVEC cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate
Lane 4: SH-SY5Y cell lysate
Recombinant Rabbit monoclonal primary
Recombinant UBC9 Monoclonal Antibody (ET1610-21)
HUVEC, A431, HepG2, human kidney tissue, mouse testis tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
SUMO-conjugating enzyme UBC9
Belongs to the ubiquitin-conjugating enzyme family.
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.