Positive control:
Lane 1: HUVEC cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate
Lane 4: SH-SY5Y cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
CHIP
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant UBC9 Monoclonal Antibody (ET1610-21)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
HUVEC cell lysate, HepG2 cell lysate, Hela cell lysate, SH-SY5Y cell lysate, A431, HepG2, human kidney tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC0534
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
18 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500- 1:1,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
UBC9
SYNONYMS
C358B7.1 antibody; p18 antibody; SUMO 1 protein ligase antibody; SUMO conjugating enzyme UBC9 antibody; SUMO-conjugating enzyme UBC9 antibody; SUMO-protein ligase antibody; SUMO1 protein ligase antibody; UBC9 antibody; UBC9_HUMAN antibody; UBCE9 antibody; Ube2i antibody; Ubiquitin carrier protein 9 antibody; Ubiquitin carrier protein antibody; Ubiquitin carrier protein I antibody; Ubiquitin conjugating enzyme 9 antibody; Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody; Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody; Ubiquitin conjugating enzyme UbcE2A antibody; Ubiquitin like protein SUMO 1 conjugating enzyme antibody; Ubiquitin protein ligase E2I antibody; Ubiquitin-conjugating enzyme E2 I antibody; Ubiquitin-protein ligase I antibody
SEQUENCE SIMILARITIES
Belongs to the ubiquitin-conjugating enzyme family.
TISSUE SPECIFICITY
Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation.