PRODUCT CODE: ET1612-65

Recombinant Tyrosine Hydroxylase Monoclonal Antibody (ET1612-65)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Tyrosine Hydroxylase on different lysates using anti-Tyrosine Hydroxylase antibody at 1/50,000 dilution.<br />
 Positive control: <br />
 Lane 1: PC-12 <br />
 Lane 2: Mouse brain
  • Western blot analysis of Tyrosine Hydroxylase on different lysates using anti-Tyrosine Hydroxylase antibody at 1/50,000 dilution.<br />
 Positive control: <br />
 Lane 1: PC-12 <br />
 Lane 2: Mouse brain
  • ICC staining Tyrosine Hydroxylase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tyrosine Hydroxylase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with Tyrosine Hydroxylase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was use as the secondary antibody
Western blot analysis of Tyrosine Hydroxylase on different lysates using anti-Tyrosine Hydroxylase antibody at 1/50,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: Mouse brain

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Tyrosine Hydroxylase Monoclonal Antibody (ET1612-65)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, N2A, SH-SY-5Y, rat brain tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD080-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:50,000-1:100,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tyrosine Hydroxylase

SYNONYMS

Dystonia 14 antibody; DYT14 antibody; DYT5b antibody; EC 1.14.16.2 antibody; OTTHUMP00000011225 antibody; OTTHUMP00000011226 antibody; ple antibody; Protein Pale antibody; TH antibody; The antibody; TY3H_HUMAN antibody; TYH antibody; Tyrosine 3 hydroxylase antibody; Tyrosine 3 monooxygenase antibody; Tyrosine 3-hydroxylase antibody; Tyrosine 3-monooxygenase antibody; Tyrosine hydroxylase antibody

SEQUENCE SIMILARITIES

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

TISSUE SPECIFICITY

Mainly expressed in the brain and adrenal glands.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson’s disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds.