PRODUCT CODE: ET1704-18

Recombinant Tyrosinase Monoclonal Antibody (ET1704-18)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

ICC staining Tyrosinase in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tyrosinase in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tyrosinase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.
ICC staining Tyrosinase in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Tyrosinase Monoclonal Antibody (ET1704-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, B16F1, MCF-7, human liver tissue, human stomach carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA52-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

80 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Tyrosinase

SYNONYMS

ATN antibody; CMM8 antibody; LB24 AB antibody; LB24-AB antibody; Monophenol monooxygenase antibody; OCA1 antibody; OCA1A antibody; OCAIA antibody; Oculocutaneous albinism IA antibody; SHEP3 antibody; SK29 AB antibody; SK29-AB antibody; Tumor rejection antigen AB antibody; TYR antibody; TYRO_HUMAN antibody; tyrosinase (oculocutaneous albinism IA) antibody; Tyrosinase antibody

SEQUENCE SIMILARITIES

Belongs to the tyrosinase family.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.

SUBCELLULAR LOCATION

Melanosome membrane, Melanosome.

FUNCTION

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone. Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity.