PRODUCT CODE: ET1704-81

Recombinant TXNRD1 Monoclonal Antibody (ET1704-81)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TXNRD1 on Hela (1) and human liver tissue lysate (2) using anti-TXNRD1 antibody at 1/1,000 dilution.
  • Western blot analysis of TXNRD1 on Hela (1) and human liver tissue lysate (2) using anti-TXNRD1 antibody at 1/1,000 dilution.
  • ICC staining TXNRD1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TXNRD1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-TXNRD1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with TXNRD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of TXNRD1 on Hela (1) and human liver tissue lysate (2) using anti-TXNRD1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TXNRD1 Monoclonal Antibody (ET1704-81)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human liver tissue lysate, Hela, HUVEC, SH-SY5Y, mouse testes tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA11-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

TXNRD1

SYNONYMS

cytoplasmic antibody; Gene associated with retinoic and IFN-induced mortality 12 protein antibody; Gene associated with retinoic and interferon-induced mortality 12 protein antibody; Gene associated with retinoid IFN induced mortality 12 protein antibody; GRIM 12 antibody; GRIM-12 antibody; GRIM12 antibody; KDRF antibody; KM 102 derived reductase like factor antibody; KM-102-derived reductase-like factor antibody; MGC9145 antibody; Oxidoreductase antibody; Thioredoxin reductase 1 antibody; Thioredoxin reductase 1 cytoplasmic antibody; Thioredoxin reductase GRIM 12 antibody; Thioredoxin reductase TR1 antibody; TR 1 antibody; TR antibody; TR1 antibody; TRXR 1 antibody; TRXR1 antibody; TRXR1_HUMAN antibody; TXNR antibody; TXNRD 1 antibody; Txnrd1 antibody

SEQUENCE SIMILARITIES

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

TISSUE SPECIFICITY

Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.

POST-TRANSLATIONAL MODIFICATION

The N-terminus of isoform 5 is blocked.; ISGylated.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx reductases (TrxR1 and TrxR2) are ubiquitous Ly noble flavoproteins that catalyze the NADPH-dependent reduction of Trx as well as several other oxidized cellular components. Mammalian Trx reductases are a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val- Asn-Val-Gly-Cys. TrxR1 and TrxR2 are also involved in the prevention of oxidative stress. Inhibition of TrxR activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases.

CITATIONS

  • Yu, Jia-Tian et al.

    Up-regulation of antioxidative proteins TRX1, TXNL1 and TXNRD1 in the cortex of PTZ kindling seizure model mice. | PloS One [2019]