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PRODUCT CODE: ET1703-83

Recombinant TTR Monoclonal Antibody (ET1703-83)

  • Recombinant

Applications

  • WB

  • IP

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of TTR on different cells lysates using anti-TTR antibody at 1/500 dilution.<br /> Positive control: <br /> Line 1: Human brain <br /> Line 2；Human lung
  • Western blot analysis of TTR on different cells lysates using anti-TTR antibody at 1/500 dilution.<br /> Positive control: <br /> Line 1: Human brain <br /> Line 2；Human lung
  • ICC staining TTR in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TTR in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TTR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- TTR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-TTR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TTR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TTR antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with TTR antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of TTR on different cells lysates using anti-TTR antibody at 1/500 dilution.
Positive control:
Line 1: Human brain
Line 2；Human lung

Applications

  • WB

  • IP

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TTR Monoclonal Antibody (ET1703-83)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human liver tissue lysate, human lung tissue lysate, A549, MCF-7, human liver carcinoma tissue, human liver tissue, human thyroid tissue, human kidney tissue, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

16 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P02766

PROTEIN NAME

TTR

SYNONYMS

Alternative names Amyloid polyneuropathy antibody; Amyloidosis I antibody; ATTR antibody; Carpal tunnel syndrome 1 antibody; CTS antibody; CTS1 antibody; Dysprealbuminemic euthyroidal hyperthyroxinemia antibody; Dystransthyretinemic hyperthyroxinemia antibody; Epididymis luminal protein 111 antibody; HEL111 antibody; HsT2651 antibody; PALB antibody; Prealbumin amyloidosis type I antibody; Prealbumin antibody; Prealbumin Thyroxine-binding antibody; Senile systemic amyloidosis antibody; TBPA antibody; Thyroxine binding prealbumin antibody; Transthyretin antibody; TTHY_HUMAN antibody; TTR antibody; TTR protein antibod

SEQUENCE SIMILARITIES

Belongs to the transthyretin family.

TISSUE SPECIFICITY

Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

POST-TRANSLATIONAL MODIFICATION

Not glycosylated under normal conditions. Following unfolding, caused for example by variant AMYL-TTR 'Gly-38', the cryptic Asn-118 site is exposed and glycosylated by STT3B-containing OST complex, leading to its degradation by the ER-associated degradation (ERAD) pathway.

SUBCELLULAR LOCATION

Secreted.Cytoplasm.

FUNCTION

Prealbumin, also designated transthyretin, is a major thyroid-hormone binding protein involved in transporting thyroxine from the bloodstream to the brain. Prealbumin is located in the cytoplasm and in the vesicles of developing rat brain cells, and is thought to be transported there from the cerebrospinal fluid via endocytosis. Sequence variants of this protein have been identified in amyloid fibrils from patients with familial amyloidotic polyneuropathy (FAP), the most common form of hereditary systemic amyloidosis. Although the biologically active form of Prealbumin is a tetramer, the amyloidogenic intermed-iate is thought to be a monomeric species. Prealbumin also binds to the retinol carrier protein RBP (retinol-binding protein). The gene encoding Prealbumin maps to human chromosome 18q12.1.