Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: Hela cell lysate
Applications
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WB
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ICC
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IF
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IHC-P
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FC
REACTIVITY
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Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant TSG101 Monoclonal Antibody (ET1701-59)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
NIH/3T3 cell lysate, Hela cell lysate, Hela, A431, SW480, human kidney tissue, mouse colon tissue, mouse kidney tissue, human colon carcinoma tissue, K562.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ0900
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
44 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:100-1:500
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IHC-P
-
1:50-1:200
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FC
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1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
TSG101
SYNONYMS
ESCRT I complex subunit TSG101 antibody; ESCRT-I complex subunit TSG101 antibody; TS101_HUMAN antibody; TSG 10 antibody; TSG 101 antibody; TSG10 antibody; Tsg101 antibody; Tumor susceptibility gene 10 antibody; Tumor susceptibility gene 101 antibody; Tumor susceptibility gene 101 protein antibody; Tumor susceptibility protein antibody; Tumor susceptibility protein isoform 3 antibody; VPS 23 antibody; VPS23 antibody
SEQUENCE SIMILARITIES
Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
TISSUE SPECIFICITY
Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
POST-TRANSLATIONAL MODIFICATION
Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane, Late endosome membrane.
FUNCTION
The transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.
CITATIONS
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Bai, Xuebing et al.
In vivo multivesicular bodies and their exosomes in the absorptive cells of the zebrafish (Danio Rerio) gut. | Fish & Shellfish Immunology [2019]
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IHC
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IF
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zebrafish
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Citation
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Vistro, Waseem Ali et al.
In Vivo Multivesicular Body and Exosome Secretion in the Intestinal Epithelial Cells of Turtles During Hibernation. | Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [2019]
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IHC
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IF
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Chinese soft-shelled turtles
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Citation
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