PRODUCT CODE: ET7106-66

Recombinant TRP1 Monoclonal Antibody (ET7106-66)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of TRP1 on melanoma tissue lysate using anti-TRP1 antibody at 1/500 dilution.
  • Western blot analysis of TRP1 on melanoma tissue lysate using anti-TRP1 antibody at 1/500 dilution.
  • ICC staining TRP1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TRP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-TRP1 antibody. Counter stained with hematoxylin.
Western blot analysis of TRP1 on melanoma tissue lysate using anti-TRP1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TRP1 Monoclonal Antibody (ET7106-66)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, HUVEC, human skin tissue, Melanoma tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU36-48

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

5,6-dihydroxyindole-2-carboxylic acid oxidase

GENE NAME

TYRP1

SYNONYMS

DHICA oxidase, TRP, TRP-1, TRP1, TYRP1, CAS2, TYRP, TYRRP

SEQUENCE SIMILARITIES

Belongs to the tyrosinase family.

TISSUE SPECIFICITY

Pigment cells.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.

SUBCELLULAR LOCATION

Melanosome membrane; Single-pass type I membrane protein. Melanosome. Note=Located to mature stage III and IV melanosomes and apposed endosomal tubular membranes. Transported to pigmented melanosomes by the BLOC-1 complex. Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38.

FUNCTION

Plays a role in melanin biosynthesis. Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+). May regulate or influence the type of melanin synthesized. Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (By similarity).