PRODUCT CODE: ET1612-52

Recombinant TRK fused gene Monoclonal Antibody (ET1612-52)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TRK fused gene on different lysates using anti-TRK fused gene antibody at 1/5,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: Hela
  • Western blot analysis of TRK fused gene on different lysates using anti-TRK fused gene antibody at 1/5,000 dilution.<br />
 Positive control: <br />
 Lane 1: 293T <br />
 Lane 2: Hela
  • ICC staining TRK fused gene in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TRK fused gene in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TRK fused gene in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.
Western blot analysis of TRK fused gene on different lysates using anti-TRK fused gene antibody at 1/5,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TRK fused gene Monoclonal Antibody (ET1612-52)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T cell lysate, Hela cell lysate, Hela, SKOV-3, A549, human tonsil tissue, mouse colon tissue, mouse kidney tissue, human lung carcinoma tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD082-0

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

TRK fused gene

SYNONYMS

FLJ36137 antibody; HMSNP antibody; OTTHUMP00000214045 antibody; OTTHUMP00000214046 antibody; OTTHUMP00000214047 antibody; OTTHUMP00000214048 antibody; Protein TFG antibody; SPG57 antibody; TF6 antibody; TFG antibody; TFG_HUMAN antibody; TRK fused antibody; TRK fused gene antibody; TRK fused gene protein antibody; TRK-fused gene protein antibody; TRKT3 antibody; TRKT3 oncogene antibody

TISSUE SPECIFICITY

Ubiquitous.

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

Oncogenic rearrangements of the NTRK1 gene, which encodes the Trk A protein, are frequently detected in thyroid carcinomas. Such rearrangements fuse the NTRK1 tyrosine kinase domain to 5'-end sequences of different genes. TRK-T3 contains 1,412 nucleotides of NTRK1 preceded by 598 nucleotides belonging to TFG (TRK-fused gene), a ubiquitously expressed gene located on chromosome 3. The TRK-T3 protein within the TFG region contains a coiled-coil motif that gives the oncoprotein the capability to form complexes. The cytoplasmic TRK-T3 protein binds to and phosphorylates the Shc and SNT1/FRS2 adaptor proteins, both of which are involved in coupling the receptor tyrosine kinase to the mitogen-activated protein kinase pathway by recruiting Grb2/SOS. SHP-1 also interacts with and down-regulates TRK-T3.