PRODUCT CODE: ET1705-68

Recombinant TMEM173 Monoclonal Antibody (ET1705-68)

  • Recombinant

Applications

  • WB

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

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Western blot analysis of TMEM173 on U937 (1) and 293 (2) cell lysate using anti-TMEM173 antibody at 1/1,000 dilution.
  • Western blot analysis of TMEM173 on U937 (1) and 293 (2) cell lysate using anti-TMEM173 antibody at 1/1,000 dilution.
  • Flow cytometric analysis of THP-1 cells with TMEM173 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of TMEM173 on U937 (1) and 293 (2) cell lysate using anti-TMEM173 antibody at 1/1,000 dilution.

Applications

  • WB

  • FC

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TMEM173 Monoclonal Antibody (ET1705-68)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

U937, 293, THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM03-47

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

TMEM173

SYNONYMS

endoplasmic reticulum IFN stimulator antibody; Endoplasmic reticulum interferon stimulator antibody; ERIS antibody; FLJ38577 antibody; hMITA antibody; hSTING antibody; Mediator of IRF3 activation antibody; MITA antibody; Mitochondrial mediator of IRF3 activation antibody; MPYS antibody; N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody; NET23 antibody; Stimulator of interferon genes antibody; Stimulator of interferon genes protein antibody; STING antibody; TM173_HUMAN antibody; Tmem173 antibody; Transmembrane protein 173 antibody

SEQUENCE SIMILARITIES

Belongs to the STING family.

TISSUE SPECIFICITY

Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by TBK1 leads to activation and production of IFN-beta. Following cyclic nucleotide (c-di-GMP or cGAMP)-binding, activation and translocation from the endoplasmic reticulum, STING1 is phosphorylated by TBK1 at Ser-366 in the pLxIS motif. The phosphorylated pLxIS motif constitutes an IRF3-binding motif, leading to recruitment of the transcription factor IRF3 to induce type-I interferons and other cytokines. Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity).; Ubiquitinated. Ubiquitinated via 'Lys-63'-linked ubiquitin chains in response to double-stranded DNA treatment, leading to relocalization to autophagosomes and subsequent degradation; this process is dependent on SQSTM1 (By similarity). 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation. 'Lys-11'-linked polyubiquitination at Lys-150 by RNF26 leads to stabilize STING1: it protects STING1 from RNF5-mediated 'Lys-48'-linked polyubiquitination.

SUBCELLULAR LOCATION

Cytoplasm, membrane.

FUNCTION

Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.