PRODUCT CODE: ET1703-30

Recombinant TLR5 Monoclonal Antibody (ET1703-30)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TLR5 on Hela cells lysates using anti-TLR5 antibody at 1/500 dilution.
  • Western blot analysis of TLR5 on Hela cells lysates using anti-TLR5 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TLR5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-TLR5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomack tissue using anti-TLR5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TLR5 antibody. Counter stained with hematoxylin.
Western blot analysis of TLR5 on Hela cells lysates using anti-TLR5 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TLR5 Monoclonal Antibody (ET1703-30)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, human liver tissue, human spleen tissue, mouse testis tissue, mouse prostate tissue, mouse stomack tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-88

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

97 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Toll-like receptor 5

GENE NAME

TLR5

SYNONYMS

TLR5

SEQUENCE SIMILARITIES

Belongs to the Toll-like receptor family.

TISSUE SPECIFICITY

Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.; Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein.

FUNCTION

Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).