PRODUCT CODE: ET1703-81

Recombinant TIMP2 Monoclonal Antibody (ET1703-81)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of TIMP2 on K562 cells lysates using anti-TIMP2 antibody at 1/500 dilution.
  • Western blot analysis of TIMP2 on K562 cells lysates using anti-TIMP2 antibody at 1/500 dilution.
  • Flow cytometric analysis of Hela cells with TIMP2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of TIMP2 on K562 cells lysates using anti-TIMP2 antibody at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TIMP2 Monoclonal Antibody (ET1703-81)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM87-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Metalloproteinase inhibitor 2

GENE NAME

TIMP2

SYNONYMS

TIMP-2, TIMP2

SEQUENCE SIMILARITIES

Belongs to the protease inhibitor I35 (TIMP) family.

POST-TRANSLATIONAL MODIFICATION

The activity of TIMP2 is dependent on the presence of disulfide bonds.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.