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PRODUCT CODE: ET1705-24

Recombinant Thymidylate Synthase Monoclonal Antibody (ET1705-24)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Thymidylate Synthase on different cell lysate using anti-Thymidylate Synthase antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: Hela <br /> Lane 2: Jurkat <br /> Lane 3: Raji <br /> Lane 4: Mouse testes<br /> Lane 5: Mouse brain<br /> Lane 6: Mouse spleen
  • Western blot analysis of Thymidylate Synthase on different cell lysate using anti-Thymidylate Synthase antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: Hela <br /> Lane 2: Jurkat <br /> Lane 3: Raji <br /> Lane 4: Mouse testes<br /> Lane 5: Mouse brain<br /> Lane 6: Mouse spleen
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat bone tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Thymidylate Synthase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Thymidylate Synthase on different cell lysate using anti-Thymidylate Synthase antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: Raji
Lane 4: Mouse testes
Lane 5: Mouse brain
Lane 6: Mouse spleen

Applications

  • WB

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Thymidylate Synthase Monoclonal Antibody (ET1705-24)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, Jurkat, Raji, mouse thymus, rat bone tissue, human tonsil tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM33-20

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

36 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P04818

PROTEIN NAME

Thymidylate Synthase

SYNONYMS

d TMP synthase antibody; EC 2.1.1.45 antibody; HsT422 antibody; MGC88736 antibody; OTTHUMP00000162195 antibody; Thymidylate synthase antibody; Thymidylate synthetase antibody; TMS antibody; TS antibody; TSase antibody; Tyms antibody; TYMS protein antibody; Tyms thymidylate synthetase antibody; TYSY_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the thymidylate synthase family.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Mitochondrion.

FUNCTION

Thymidylate synthase (TS), also designated TYMS, TMS, TSase and HsT422, uses 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor in the synthesis of 2'-deoxythymidine-5'-monophosphate (dTMP), an essential precursor for DNA biosynthesis. TS is an RNA-binding protein that can interact with its own mRNA. The TS/mRNA ribonucleoprotein complex can also associate with a number of other cellular mRNAs, including those corresponding to the p53 tumor suppressor gene and the Myc family of transcription factors. Inhibition of DNA replication and cell death resulting from thymidine depletion occurs when TS enzyme activity is inhibited with substrate or cofactor analogs, making the TS enzyme an important target for chemotherapy. Cancer cells are sensitive to thymidine depletion, as they multiply rapidly.