PRODUCT CODE: ET1702-90

Recombinant Thioredoxin Monoclonal Antibody (ET1702-90)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: 293T
  • Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: 293T
  • Flow cytometric analysis of Hela cells with TRX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Thioredoxin Monoclonal Antibody (ET1702-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF101-8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

12 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Thioredoxin

GENE NAME

TXN

SYNONYMS

TRX1, Trx, ADF, SASP, TXN

SEQUENCE SIMILARITIES

Belongs to the thioredoxin family.

POST-TRANSLATIONAL MODIFICATION

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.; In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Secreted. Note=Translocates from the cytoplasm into the nucleus after phorbol 12-myristate 13-acetate induction (PMA). Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. Secreted by a leaderless secretory pathway.

FUNCTION

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.; ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).