Positive control:
Lane 1: K562 cell lysate
Lane 2: Hela cell lysate
Applications
-
WB
-
IP
-
ICC
-
IF
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
-
Zebrafish
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant TDP43 Monoclonal Antibody (ET1703-74)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
K562 cell lysate, Hela cell lysate, 293T, Hela, SH-SY5Y, human spleen tissue, human pancreas tissue, mouse placenta tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM51-10
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
45 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC/IF
-
1:100-1:500
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
-
IP
-
1:10-1:50
TARGET
UNIPROT #
PROTEIN NAME
TDP43
SYNONYMS
ALS10 antibody; OTTHUMP00000002171 antibody; OTTHUMP00000002172 antibody; OTTHUMP00000002173 antibody; TADBP_HUMAN antibody; TAR DNA binding protein 43 antibody; TAR DNA binding protein antibody; TAR DNA-binding protein 43 antibody; TARDBP antibody; TDP 43 antibody; TDP-43 antibody; TDP43 antibody
TISSUE SPECIFICITY
Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
POST-TRANSLATIONAL MODIFICATION
Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Phosphorylated upon cellular stress.; Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.; Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
SUBCELLULAR LOCATION
Nucleus..
FUNCTION
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.