PRODUCT CODE: ET1706-48

Recombinant TCTP Monoclonal Antibody (ET1706-48)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TCTP on different lysates using anti-TCTP antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse placenta tissue<br />
 Lane 2: Mouse testis tissue<br />
 Lane 3: Hela<br />
 Lane 4: A431<br />
 Lane 5: MCF-7<br />
  • Western blot analysis of TCTP on different lysates using anti-TCTP antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse placenta tissue<br />
 Lane 2: Mouse testis tissue<br />
 Lane 3: Hela<br />
 Lane 4: A431<br />
 Lane 5: MCF-7<br />
  • ICC staining TCTP in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TCTP in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TCTP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TCTP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TCTP antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of THP-1 cells with TCTP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor&reg; 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of TCTP on different lysates using anti-TCTP antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse placenta tissue
Lane 2: Mouse testis tissue
Lane 3: Hela
Lane 4: A431
Lane 5: MCF-7

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TCTP Monoclonal Antibody (ET1706-48)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse placenta tissue lysate, mouse testis tissue, Hela, A431, MCF-7, LOVO, SH-SY5Y, human colon cancer tissue, THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU13-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Translationally-controlled tumor protein

GENE NAME

TPT1

SYNONYMS

TCTP, HRF, TPT1

SEQUENCE SIMILARITIES

Belongs to the TCTP family.

TISSUE SPECIFICITY

Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Involved in calcium binding and microtubule stabilization.