Positive control:
Lane 1: PC-12 cell lysate
Lane 2: JAR cell lysate
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant TAPA1/CD81 Monoclonal Antibody (ET1611-87)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
PC-12 cell lysate, JAR cell lysate, 293, A549, SH-SY5Y, PC-12, human liver tissue, mouse testis tissue, mouse lung tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN206-01
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
20 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC
-
1:100-1:500
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
TAPA1/CD81
SYNONYMS
26 kDa cell surface protein TAPA 1 antibody; 26 kDa cell surface protein TAPA-1 antibody; 26 kDa cell surface protein TAPA1 antibody; CD 81 antibody; CD81 antibody; CD81 antigen (target of antiproliferative antibody; 1) antibody; CD81 antigen antibody; CD81 molecule antibody; CD81_HUMAN antibody; CVID6 antibody; S5.7 antibody; TAPA 1 antibody; TAPA1 antibody; Target of the antiproliferative antibody; 1 antibody; Tetraspanin 28 antibody; Tetraspanin-28 antibody; Tetraspanin28 antibody; Tspan 28 antibody; Tspan-28 antibody; Tspan28 antibody
SEQUENCE SIMILARITIES
Belongs to the tetraspanin (TM4SF) family.
TISSUE SPECIFICITY
Expressed on B cells (at protein level). Expressed in hepatocytes (at protein level). Expressed in monocytes/macrophages (at protein level). Expressed on both naive and memory CD4-positive T cells (at protein level).
POST-TRANSLATIONAL MODIFICATION
Not glycosylated.; Likely constitutively palmitoylated at low levels. Protein palmitoylation is up-regulated upon coligation of BCR and CD9-C2R-CD81 complexes in lipid rafts.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
CD81, also called TAPA-1, is a type III transmembrane protein that is broadly expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin. CD81 is believed to be involved in both cell growth and signal transduction. It can be present as a multimolecular complex in association with CD37 and/or CD53, or on the surface of B cells in association with CD19, CD21 and/or MHC class II antigens.