PRODUCT CODE: ET1705-14

Recombinant TAK1 Monoclonal Antibody (ET1705-14)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of TAK1 on human placenta tissue lysate using anti-TAK1 antibody at 1/1,000 dilution.
  • Western blot analysis of TAK1 on human placenta tissue lysate using anti-TAK1 antibody at 1/1,000 dilution.
  • ICC staining TAK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TAK1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with TAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of TAK1 on human placenta tissue lysate using anti-TAK1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TAK1 Monoclonal Antibody (ET1705-14)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7 cell lysate, A431 cell lysate, A431, N2A, human prostate tissue, mouse stomach tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM73-19

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

67 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC/IF

  • 1:50-1:100

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

TAK1

SYNONYMS

M3K7_HUMAN antibody; MAP3K 7 antibody; Map3k7 antibody; MEKK7 antibody; Mitogen activated protein kinase kinase kinase 7 antibody; Mitogen-activated protein kinase kinase kinase 7 antibody; TAK1 antibody; TGF beta activated kinase 1 antibody; TGF-beta-activated kinase 1 antibody; TGF1a antibody; Transforming growth factor beta activated kinase 1 antibody; Transforming growth factor-beta-activated kinase 1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

TISSUE SPECIFICITY

Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.

POST-TRANSLATIONAL MODIFICATION

Association with TAB1/MAP3K7IP1 promotes autophosphorylation at Ser-192 and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Ser-192 by PPM1B/PP2CB and at Thr-187 by PP2A and PPP6C leads to inactivation.; 'Lys-48'-linked polyubiquitination at Lys-72 is induced by TNFalpha, and leads to proteasomal degradation. Undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.; (Microbial infection) Cleaved and inactivated by the proteases 3C of coxsackievirus A16 and human enterovirus D68, allowing the virus to disrupt TRAF6-triggered NF-kappa-B induction.

SUBCELLULAR LOCATION

Cell membrane. Cytoplasm.

FUNCTION

Several serine / threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK / MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a protein with intrinsic kinase activity towards serine / threonine residues and Which is expressed expression in many human types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation is poorly understood. Two proteins put put involved in this process are Ksr-1 and Taks. Ksr-1 (kinase sup-pressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether or not in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFβ stimulation.