PRODUCT CODE: ET1605-18

Recombinant TACC3 Monoclonal Antibody (ET1605-18)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of TACC3 on different lysates using anti-TACC3 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HT29 <br />
Lane 2: Hela
  • Western blot analysis of TACC3 on different lysates using anti-TACC3 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: HT29 <br />
Lane 2: Hela
  • ICC staining TACC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining TACC3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
Western blot analysis of TACC3 on different lysates using anti-TACC3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HT29
Lane 2: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant TACC3 Monoclonal Antibody (ET1605-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HT29, Hela, HepG2, human tonsil tissue, human spleen tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY10-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

140 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Transforming acidic coiled-coil-containing protein 3

GENE NAME

TACC3

SYNONYMS

TACC3, ERIC1

SEQUENCE SIMILARITIES

Belongs to the TACC family.

SUBCELLULAR LOCATION

Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole.

FUNCTION

Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer.