PRODUCT CODE: ET1606-56

Recombinant Synaptophysin Monoclonal Antibody (ET1606-56)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.<br />
Positive control:<br />
Lane 1: Human brain<br />
Lane 2: PC-12
  • Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.<br />
Positive control:<br />
Lane 1: Human brain<br />
Lane 2: PC-12
  • ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.
Positive control:
Lane 1: Human brain
Lane 2: PC-12

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Synaptophysin Monoclonal Antibody (ET1606-56)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, N2A, human brain lysate, human pancreas tissue, mouse brain tissue, mouse pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SJ26-85

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Synaptophysin

GENE NAME

SYP

SYNONYMS

SYP

SEQUENCE SIMILARITIES

Belongs to the synaptophysin/synaptobrevin family.

TISSUE SPECIFICITY

Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.

FUNCTION

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).