PRODUCT CODE: ET1606-56

Recombinant Synaptophysin Monoclonal Antibody (ET1606-56)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Human brain<br />
 Lane 2: PC-12
  • Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Human brain<br />
 Lane 2: PC-12
  • ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.
Positive control:
Lane 1: Human brain
Lane 2: PC-12

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Synaptophysin Monoclonal Antibody (ET1606-56)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, N2A, human brain lysate, human pancreas tissue, mouse brain tissue, mouse pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SJ26-85

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Synaptophysin

SYNONYMS

Major synaptic vesicle protein p38 antibody; MRX96 antibody; MRXSYP antibody; Syn p38 antibody; Synaptophysin antibody; Syp antibody; SYPH antibody; SYPH_HUMAN antibody; SypI antibody

SEQUENCE SIMILARITIES

Belongs to the synaptophysin/synaptobrevin family.

TISSUE SPECIFICITY

Expressed in the brain, with expression in the hippocampus, the neuropil in the dentate gyrus, where expression is higher in the outer half of the molecular layer than in the inner half, and in the neuropil of CA4 and CA3.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasmic, Cell junction, synapse, synaptosome.

FUNCTION

Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings.