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PRODUCT CODE: ET1701-62

Recombinant SUZ12 Monoclonal Antibody (ET1701-62)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution.
  • Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution.
  • ICC staining SUZ12 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUZ12 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUZ12 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUZ12 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SUZ12 Monoclonal Antibody (ET1701-62)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, MCF-7, Hela, SKOV-3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ09-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

83 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

Q15022

PROTEIN NAME

SUZ12

SYNONYMS

ChET 9 protein antibody; CHET9 antibody; Chromatin precipitated E2F target 9 protein antibody; JJAZ1 antibody; Joined to JAZF1 protein antibody; KIAA0160 antibody; Polycomb protein SUZ12 antibody; Suppressor of zeste 12 homolog antibody; Suppressor of zeste 12 protein homolog antibody; SUZ12 antibody; SUZ12 polycomb repressive complex 2 subunit antibody; SUZ12_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.

TISSUE SPECIFICITY

Overexpressed in breast and colon cancer.

DEVELOPMENTAL STAGE

Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.

POST-TRANSLATIONAL MODIFICATION

Sumoylated, probably by PIAS2.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis. The mammalian PcG proteins are regulatory proteins important for Hox gene expression, axial skeleton development and the control of proliferation and survival of hematopoietic cells. By inducing changes in chromatin structure, the PcG proteins are part of a cellular memory system that is responsible for gene activity being inherited to progeny cells. PcG proteins silence gene expression through the formation of multimeric protein complexes with different compositions. Manipulating the expression-levels of various PcG proteins in mammalian cell lines results in cellular transformation, which may be a link between the chromatin-associated PcG proteins and cancer. Polycomb protein SUZ12, also designated ChET 9 protein or joined-to-JAZF1 protein, is a nuclear protein belonging to the VEFS (VRN2-EMF2-FIS2-SUZ12) family. SUZ12 has been detected at the breakpoints of a certain recurrent chromosomal translocation which has been reported in endometrial stromal sarcoma. It is a component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7.