PRODUCT CODE: ET1701-62

Recombinant SUZ12 Monoclonal Antibody (ET1701-62)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution.
  • Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution.
  • ICC staining SUZ12 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUZ12 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUZ12 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SUZ12 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of SUZ12 on MCF-7 cells lysates using anti-SUZ12 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SUZ12 Monoclonal Antibody (ET1701-62)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, MCF-7, Hela, SKOV-3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ09-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

83 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Polycomb protein SUZ12

GENE NAME

SUZ12

SYNONYMS

ChET 9 protein, SUZ12

SEQUENCE SIMILARITIES

Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.

TISSUE SPECIFICITY

Overexpressed in breast and colon cancer.

DEVELOPMENTAL STAGE

Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.

POST-TRANSLATIONAL MODIFICATION

Sumoylated, probably by PIAS2.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.