PRODUCT CODE: ET1701-45

Recombinant STAT 5A+B Monoclonal Antibody (ET1701-45)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of STAT5A+B on different lysates using anti-STAT5A+B antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: K562 <br />
Lane 2: Jurkat <br />
Lane 3: PC-12
  • Western blot analysis of STAT5A+B on different lysates using anti-STAT5A+B antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: K562 <br />
Lane 2: Jurkat <br />
Lane 3: PC-12
  • ICC staining STAT5A+B in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining STAT5A+B in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining STAT5A+B in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of NIH/3T3 cells with STAT5A+B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of STAT5A+B on different lysates using anti-STAT5A+B antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: Jurkat
Lane 3: PC-12

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant STAT 5A+B Monoclonal Antibody (ET1701-45)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, Jurkat, PC-12, HepG2, RH-35, NIH/3T3, human tonsil tissue, human spleen tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ08-78

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Signal transducer and activator of transcription 5A

GENE NAME

STAT5A

SYNONYMS

STAT5A, STAT5B

SEQUENCE SIMILARITIES

Belongs to the transcription factor STAT family.

POST-TRANSLATIONAL MODIFICATION

Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694.; ISGylated.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.

FUNCTION

Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.