PRODUCT CODE: ET1704-76

Recombinant SPHK1 Monoclonal Antibody (ET1704-76)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.
  • Western blot analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SPHK1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Raji cells with SPHK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SPHK1 Monoclonal Antibody (ET1704-76)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293 cell lysate, C2C12 cell lysate, human kidney tissue, Raji.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA31-14

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

51 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

SPHK1

SYNONYMS

SK 1 antibody; SK1 antibody; Sphingosine kinase 1 antibody; SPHK 1 antibody; SPHK antibody; Sphk1 antibody; SPHK1_HUMAN antibody; SPK antibody; SPK 1 antibody

TISSUE SPECIFICITY

Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level).

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Membrane.

FUNCTION

Sphingosine kinase (SphK or SphK1) is a key enzyme catalyzing the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP or S1P). Competitive inhibitors of SphK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. One potent inhibitor of SphK1 activity is DMS (N,N-dimethylsphingosine). SPP/SphK1 has been implicated as a signaling pathway that regulates diverse cellular functions, including cell growth, proliferation and survival. Specifically, SphK1 is involved in the signaling pathway(s) that protects human hepatocytes from the apoptotic action of TNFα. Furthermore, SPP/SphK1 may play an important role in neuronal survival by regulating activation of SAPKs and caspases. SphK1 is widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle; however, activation of SphK1 disengages cells from their liver-specific phenotype.