Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant SPHK1 Monoclonal Antibody (ET1704-76)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
293 cell lysate, C2C12 cell lysate, human kidney tissue, Raji.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA31-14
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
51 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50
TARGET
UNIPROT #
PROTEIN NAME
SPHK1
SYNONYMS
SK 1 antibody; SK1 antibody; Sphingosine kinase 1 antibody; SPHK 1 antibody; SPHK antibody; Sphk1 antibody; SPHK1_HUMAN antibody; SPK antibody; SPK 1 antibody
TISSUE SPECIFICITY
Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level).
SUBCELLULAR LOCATION
Cytoplasm. Nucleus. Membrane.
FUNCTION
Sphingosine kinase (SphK or SphK1) is a key enzyme catalyzing the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP or S1P). Competitive inhibitors of SphK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. One potent inhibitor of SphK1 activity is DMS (N,N-dimethylsphingosine). SPP/SphK1 has been implicated as a signaling pathway that regulates diverse cellular functions, including cell growth, proliferation and survival. Specifically, SphK1 is involved in the signaling pathway(s) that protects human hepatocytes from the apoptotic action of TNFα. Furthermore, SPP/SphK1 may play an important role in neuronal survival by regulating activation of SAPKs and caspases. SphK1 is widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle; however, activation of SphK1 disengages cells from their liver-specific phenotype.