PRODUCT CODE: ET1612-76

Recombinant SOX11 Monoclonal Antibody (ET1612-76)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SOX11 on human brain using anti-SOX11 antibody at 1/500 dilution.
  • Western blot analysis of SOX11 on human brain using anti-SOX11 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-SOX11 antibody. Counter stained with hematoxylin.
  • ICC staining SOX11 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SOX11 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of SOX11 on human brain using anti-SOX11 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SOX11 Monoclonal Antibody (ET1612-76)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY-5Y, N2A.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD203-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

SOX11

SYNONYMS

MRD27 antibody; SOX11 antibody; SOX11_HUMAN antibody; SRY (sex determining region Y) box 11 antibody; SRY related HMG box gene 11 antibody; SRY-box 11 antibody; Transcription factor SOX-11 antibody

TISSUE SPECIFICITY

Expressed primarily in the brain and heart, with low expression in the kidney, pancreas and muscle.

DEVELOPMENTAL STAGE

Expressed primarily in the fetal brain, with low expression in the lung, and kidney at 6-7 weeks dpc. Weak expression in the fetal heart and muscle.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The Sox family are comprised of members that are related to the mammalian sex determining gene SRY. The Sox family of genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been conserved during evolution and play key roles during animal development. Some are involved in human diseases, including sex reversal. Sox-11 (SRY (sex determining region Y)-box 11) is a 441 amino acid nuclear protein expressed mainly in the nervous system. Containing an HMG box DNA-binding domain, Sox-11 may play a critical role in the developing nervous system.