PRODUCT CODE: ET1611-89

Recombinant SOX10 Monoclonal Antibody (ET1611-89)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SOX10 on different lysates using anti-SOX10 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Mouse brain <br />
Lane 2: HepG2
  • Western blot analysis of SOX10 on different lysates using anti-SOX10 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Mouse brain <br />
Lane 2: HepG2
  • ICC staining SOX10 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SOX10 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SOX10 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of SOX10 on different lysates using anti-SOX10 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse brain
Lane 2: HepG2

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SOX10 Monoclonal Antibody (ET1611-89)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, MCF-7, RH-35, HepG2, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN20-41

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Transcription factor SOX-10

GENE NAME

SOX10

SYNONYMS

SOX10

TISSUE SPECIFICITY

Expressed in fetal brain and in adult brain, heart, small intestine and colon.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side.

FUNCTION

Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3. Transcriptional activator of MBP, via binding to the gene promoter (By similarity).