PRODUCT CODE: ET1705-58

Recombinant SMYD3 Monoclonal Antibody (ET1705-58)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat kidney<br />
 Lane 2: PC-12<br />
 Lane 3: Hela <br />
 Lane 4: Mouse spleen<br />
 Lane 5: NIH-3T3<br />
 Lane 6: Rat spleen
  • Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat kidney<br />
 Lane 2: PC-12<br />
 Lane 3: Hela <br />
 Lane 4: Mouse spleen<br />
 Lane 5: NIH-3T3<br />
 Lane 6: Rat spleen
  • ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SMYD3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat kidney
Lane 2: PC-12
Lane 3: Hela
Lane 4: Mouse spleen
Lane 5: NIH-3T3
Lane 6: Rat spleen

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SMYD3 Monoclonal Antibody (ET1705-58)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat kidney tissue lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, PC-12, NIH-3T3, HepG2, Hela, LOVO, human tonsil tissue, human colon cancer tissue, human placenta tissue, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM73-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SMYD3

SYNONYMS

bA74P14.1 (novel protein) antibody; bA74P14.1 antibody; FLJ21080 antibody; histone lysine N methyltransferase SMYD3 antibody; KMT3E antibody; MGC104324 antibody; SET and MYND domain containing 3 antibody; SET and MYND domain containing protein 3 antibody; SET and MYND domain-containing protein 3 antibody; SMYD 3 antibody; Smyd3 antibody; SMYD3 protein antibody; SMYD3_HUMAN antibody; Zinc finger MYND domain containing 1 antibody; Zinc finger MYND domain containing protein 1 antibody; Zinc finger MYND domain-containing protein 1 antibody; Zinc finger protein subfamily 3A MYND domain containing 1 antibody; Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody; ZMYND 1 antibody; ZMYND1 antibody; ZNFN3A1 antibody

SEQUENCE SIMILARITIES

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family.

TISSUE SPECIFICITY

Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histanes methyltransferase. Methylans 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC- 3 'or 5'-GAGGGG-3'.