PRODUCT CODE: ET1703-35

Recombinant SMC3 Monoclonal Antibody (ET1703-35)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Line1: HepG2 <br />
  Line2: NIH-3T3 <br />
  Line3:PC12
  • Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Line1: HepG2 <br />
  Line2: NIH-3T3 <br />
  Line3:PC12
  • ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with SMC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution.
Positive control:
Line1: HepG2
Line2: NIH-3T3
Line3:PC12

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SMC3 Monoclonal Antibody (ET1703-35)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, NIH-3T3, PC12, Hela, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse colon tissue, zebrafish tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-75

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

141 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SMC3

SYNONYMS

BAM antibody; Bamacan antibody; Basement membrane associated chondroitin proteoglycan antibody; Basement membrane-associated chondroitin proteoglycan antibody; BMH antibody; CDLS3 antibody; chondroitin sulfate proteoglycan 6 (bamacan) antibody; Chondroitin sulfate proteoglycan 6 antibody; Chromosome associated polypeptide antibody; Chromosome-associated polypeptide antibody; CSPG 6 antibody; CSPG6 antibody; hCAP antibody; Human chromosome associated polypeptide antibody; im:7142991 antibody; SMC 3 antibody; SMC protein 3 antibody; SMC-3 antibody; smc3 antibody; SMC3_HUMAN antibody; SMC3L1 antibody; Structural maintenance of chromosome 3 antibody; Structural maintenance of chromosomes 3 antibody; Structural maintenance of chromosomes protein 3 antibody; u:fb22e01 antibody; wu:fc30d07 antibody

SEQUENCE SIMILARITIES

Belongs to the SMC family. SMC3 subfamily.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation.; Phosphorylated at Ser-1083 in a SPO11-dependent manner.; Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.

SUBCELLULAR LOCATION

Nucleus. Chromosome.

FUNCTION

The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology.