PRODUCT CODE: ET7107-12

Recombinant SMARCC1 Monoclonal Antibody (ET7107-12)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

  • IP

REACTIVITY

  • Human

  • Rat

Western blot analysis of SMARCC1 on different cell lysates using anti-SMARCC1 at 1/500 dilution.<br />
  Positive control:<br />
  Line 1: Mouse thymus tissue <br />
  Line 2: HL-60
  • Western blot analysis of SMARCC1 on different cell lysates using anti-SMARCC1 at 1/500 dilution.<br />
  Positive control:<br />
  Line 1: Mouse thymus tissue <br />
  Line 2: HL-60
  • ICC staining SMARCC1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SMARCC1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with SMARCC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SMARCC1 on different cell lysates using anti-SMARCC1 at 1/500 dilution.
Positive control:
Line 1: Mouse thymus tissue
Line 2: HL-60

Applications

  • WB

  • ICC

  • IF

  • FC

  • IP

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SMARCC1 Monoclonal Antibody (ET7107-12)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse thymus tissue, HL-60, 293T, mouse testis tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB43-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

123 kDa (Predicted band size) 150 kDa (Observed band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

SMARCC1

SYNONYMS

AI115498 antibody; BAF 155 antibody; BAF155 antibody; BRG 1 associated factor 155 antibody; BRG1 associated factor 155 antibody; BRG1-associated factor 155 antibody; Chromatin remodeling complex BAF155 subunit antibody; CRACC 1 antibody; CRACC1 antibody; Mammalian chromatin remodeling complex BRG 1 associated factor 155 antibody; Mammalian chromatin remodeling complex BRG1 associated factor 155 antibody; Rsc 8 antibody; Rsc8 antibody; SMARC C1 antibody; SMARCC 1 antibody; SMARCC1 antibody; SMRC1_HUMAN antibody; SRG 3 antibody; SRG3 antibody; SWI 3 antibody; SWI/SNF complex 155 kDa subunit antibody; SWI/SNF complex subunit SMARCC1 antibody; SWI/SNF related matrix associated actin dependent regulator of chromatin c1 antibody; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 antibody; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 antibody; SWI3 antibody

SEQUENCE SIMILARITIES

Belongs to the SMARCC family.

TISSUE SPECIFICITY

Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on undefined residues at the G2/M transition by ERK1 and other kinases. This may contribute to cell cycle specific inactivation of remodeling complexes containing the phosphorylated protein.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes.